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KMID : 0364219960390030231
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Korean Journal of Zoology
1996 Volume.39 No. 3 p.231 ~ p.238
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Mutational Analysis of K-ras and p53 Genes in Human Lung and Pancreatic Carcinoma Cell Lines
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Jung Kiong-Lee
Jung Noh-Pal
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Abstract
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Several types of human lung and pancreatic carcinoma cell lines were cultured and their chromosomal DNAs were extracted. These DNAs were then partially amplified by PCR (Polymerase Chain Reaction) and sequenced to analyze the types and frequency of mutations, and their possible relation in the oncogene, K-ras and suppressor gene, p53. Regardless of the cell line origin, 81% were found to possess at least one mutation. Among the cell lines analyzed, 54.5% of the mutations were found in either K-ras or p53. Except for one nonsense mutation, all mutations were missense with either base insertions or substitutions. Furthermore, besides the p53 codons Known to be mutated simultaneously with' ras to enhance tumor growth, p53 164-165 and 248 were found to be mutated simultaneously with K-ms. Regardless of the site of p53 mutation, all K-ras mutations found in these cases occurred at exon 1, codon 12.
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KEYWORD
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Ras, p53, PCR, DNA Sequencing
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